![]() ![]() pingshaense WP08 with high toxicity, was isolated from cadavers of M. are broad-spectrum fungi, which have been demonstrated to effectively control more than 200 pest populations including the underground wireworms, Agriotes obscurus, Limonius californicus and Hypnoidus bicolor. Entomopathogenic fungi are naturally available infectious agents that can be used as complementary biocontrol measures. However, due to pesticide resistance, environment pollution, food safety issues and various other negative impacts, alternative pest control strategies are urgently needed. Current measures to control this pest include the use of conventional insecticides, such as carbofuran and clothianidin. cribricollis are predominant in southern China and account to about 3 wireworms per kilogram of cultivated bamboo and even 19 wireworms have been found in one bamboo shoot. Recently, bamboo wireworm larvae have become rampant in southern China due to the transformation of tillage system and cultivation of a single fast-growing bamboo species. Larvae not only feed on bamboo shoots but also injure root systems, reducing bamboo shoot outputs and commodity value, thus, severely affecting the sustainable regeneration of bamboo forests. ![]() and are distributed worldwide The bamboo wireworm ( Melanotus cribricollis Coleoptera: Elateridae) pose danger to bamboos, especially the Phyllostachys spp. Larvae of the beetle superfamily, Elateroidea, are agricultural pests that feed on germinating seeds, seedlings, tubers, root tubers, etc. All other relevant data are within the paper and its Supporting Information files.įunding: This work was supported by “The Fundamental Research Funds for the Central Non-profit Research Institution of CAF (CAFYBB2016SY012)” and “The Special Fund for Forestry Scientific Research in the Public Interest (201304403).”Ĭompeting interests: The authors have declared that no competing interests exist. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.ĭata Availability: The RNA-Seq sequencing data of both Melanotus cribricollis larvae and Metarhizium anisopliae WP08 strain have been uploaded to the Sequence Read Archive (SRA) database at NCBI the relevant accession numbers are as follows: RJNA417752, SRP124912, PRJNA417871 and SRP124980. Received: AugAccepted: DecemPublished: January 16, 2018Ĭopyright: © 2018 Ye et al. PLoS ONE 13(1):Įditor: Irene Söderhäll, Uppsala Universitet, SWEDEN infected with a fungus, and it could provide insights to further explore insect–fungi interaction mechanisms.Ĭitation: Ye B-h, Zhang Y-b, Shu J-p, Wu H, Wang H-j (2018) RNA-sequencing analysis of fungi-induced transcripts from the bamboo wireworm Melanotus cribricollis (Coleoptera: Elateridae) larvae. To our knowledge, this was the first report of transcriptome analysis of Melanotus spp. cribricollis larvae are initiated to protect against the more serious negative effects caused by fungal infection. The results indicated that, multiple defense mechanisms of M. The de novo assembly, functional annotation, sequence comparison of four insect species, and analysis of DEGs, enriched pathways, GO terms and immune related candidate genes were operated. The reliability of the RNA-Seq data was also validated through qRT-PCR experiment. pingshaense WP08 was also performed for data separation of those two non-model species. In addition, the transcriptome sequencing of M. pingshaense WP08 or not, using high-throughput next generation sequencing technology. Here, we analyzed the whole transcriptome of M. So far, the genetic backgrounds of both M. cribricollis larvae cadavers named Metarhizium pingshaense WP08 showed high pathogenic efficacy indoors, indicated that the fungus could be used as a bio-control measure. Previously, a new entomopathogenic fungal strain isolated from M. However, there is currently no effective control measure to limit the population of this underground pest. Wanna stroll there and check it out? Need to search some specific places, I've even got a list of where there could still be unlooted stuff.Larvae of Melanotus cribricollis, feed on bamboo shoots and roots, causing serious damage to bamboo in Southern China. I wanna know if there's anything left there so I could gather a group for it, or if everything's already looted. The reserve base's got several soldier barracks, and usually, they're supposed to have an arsenal and a storage room in them. Salam, brother! Come in! Got a task for you. ![]()
0 Comments
Leave a Reply. |
AuthorWrite something about yourself. No need to be fancy, just an overview. ArchivesCategories |